Quadruplex Nucleic Acids by Jonathan B. Chaires & David Graves

Author:Jonathan B. Chaires & David Graves
Language: eng
Format: epub
Publisher: Springer Berlin Heidelberg, Berlin, Heidelberg

Protoporphyrin IX (PPIX) is known as a photodynamic agent in cancer therapy, able to interact with various proteins. On the tracks of NMM, binding of PPIX against various G4-DNA (TBA, human telomeric, or Oxy28) has been investigated [115]. The emission spectrum of PPIX is typical of a porphyrin compound with a dual band in the 600–700 nm range. The fluorescence enhancement efficiencies observed with different G4-DNA are from 16.2 to 17.9-fold, whilst the binding constants are rather different (K d from 0.04–50 μM). In the same work, PPIX has also been used as a “sensor” of parallel quadruplexes formed in K+ conditions as the fluorescence increases rapidly when the K+ concentration rises from 2 to 100 mM. The fluorescence properties of Zn-PPIX have also been used to monitor the lead-driven interconversion between a duplex and a G4 form acting as components of a DNA nanodevice [116].

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